Factor VIII Recombination After Dissociation by CaCl2

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Factor VIII Recombination After Dissociation by CaC 12 ( canine plasma fractions / antihemophilic factor / gel chromatography / molecular weight ) HERBERT

Factor VIII is a large protein molecule of molecular weight 2,000,000 or larger that elutes in the void volume on agarose gel chromatography. It has been shown previously that high concentrations of alkali halides and, more specifically, 0.25 M Ca2+ dissociate the molecule into a large carrier protein and a small fragment that retains the factor VIII activity. Factor VIII was prepared from norm...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1973

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.70.8.2326